MP08-11 ENHANCED NEUROGENIC ACTIVITY AND NERVE DENSITY IN SMOOTH MUSCLE STRIPS TAKEN FROM CLOSER TO THE SEROSAL SIDE OF THE HUMAN AND DOG BLADDER

You have accessJournal of UrologyBladder & Urethra: Anatomy, Physiology Pharmacology (MP08)1 Sep 2021MP08-11 ENHANCED NEUROGENIC ACTIVITY AND NERVE DENSITY IN SMOOTH MUSCLE STRIPS TAKEN FROM CLOSER TO THE SEROSAL SIDE OF HUMAN DOG BLADDER Nagat Frara, Mary F. Barbe, Dania Giaddui, Alan S. Braverman, Lucas J. Hobson, Geneva E. Cruz, Mamta Amin, and Michael R. Ruggieri FraraNagat Frara More articles by this author , BarbeMary Barbe GiadduiDania Giaddui BravermanAlan Braverman HobsonLucas Hobson CruzGeneva Cruz AminMamta Amin RuggieriMichael View All Author Informationhttps://doi.org/10.1097/JU.0000000000001981.11AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION OBJECTIVE: In normal dog human bladders, we aimed investigate regional variations in muscle strip responses different neurogenic myogenic stimulations; with the assumption that smooth bladder has similar contractile properties throughout wall thickness. METHODS: Bladders were harvested from seven male dogs another three obtained organ transplant donors; one two females. Smooth strips dissected dorsal ventral aspects body at least 1 cm above ureteral orifices. Mucosa was removed adjacent mucosa separate serosa. Maximal electric field stimulation (EFS) 120 mM potassium chloride (KCl) determined. After washing re-equilibration, 30μM muscarinic receptor agonist bethanechol At end each experiment, collected, fixed stained for axonal marker protein gene product 9.5 (PGP9.5) assess density. RESULTS: serosa showed more expression PGP9.5 greater axon density, than close mucosa. While EFS-induced contractions significantly increased sub-serosal compared sub-mucosal strips, EFS not between bladders. induced either KCl or immediately beneath However, mucosal serosal side. CONCLUSIONS: Much previous literature on incorporates implicit versus across thickness respond similarly pharmacological agents. We observed first time bladder, stimulators taken lumen closer outer surface. The increase density nerves is surface suggesting higher numbers intramural axons. This finding may important implications understanding physiology biophysics emptying. Source Funding: NINDS R01NS070267 © 2021 American Urological Association Education Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e154-e154 Advertisement Copyright Permissions© Inc.MetricsAuthor Information Expand Loading ...